Acid-Base Transport and Control in Locust Hindgut: Artefacts Caused by Short-Circuit Current
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چکیده
منابع مشابه
Acid-base Transport and Control in Locust Hindgut: Artefacts Caused by Short-circuit Current
1. The commonly used method of passing short-circuit current (/sc) across insect epithelia through Ag-AgCl electrodes, without the use of salt bridges, leads to significant OH~ production at the cathode (lumen side) when high currents are applied. 2. The alkalization of the lumen previously reported when cyclic AMP was added to short-circuited locust hindgut is a result of this phenomenon rathe...
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The effect of palmitic acid (PA) and oleic acid (OA) on electrical parameters of planar membranes was studied. We found a substantial difference between the effects of PA and OA on proton transfer. PA induced a small increase in conductance, requiring a new technique for estimating proton-mediated currents across low-conductance planar bilayers in which an electrometer is used to measure the tr...
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Artefacts which occur during the processing of small biopsy specimens can cause sufficient tissue distortion to impair interpretation and can be a considerable source of nuisance. Triangular artefacts were noted in renal and liver biopsy specimens which were caused by foam sponges in embedding cassettes. Scanning electron microscopic examination of the sponges showed they comprised a mesh of sc...
متن کاملTissue artefacts caused by sponges
Artefacts which occur during the processing of small biopsy specimens can cause sufficient tissue distortion to impair interpretation and can be a considerable source of nuisance. Triangular artefacts were noted in renal and liver biopsy specimens which were caused by foam sponges in embedding cassettes. Scanning electron microscopic examination of the sponges showed they comprised a mesh of sc...
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1. Recta of desert locusts were short-circuited and depleted of endogenous substrates by exposing them to saline containing cyclic AMP but no metabolites. Individual substrates were then added to substrate-depleted recta and the change in short-circuit current (/sc) monitored. 2. Proline or glucose (50 mil) caused by far the largest increase in 780 of all substrates tested. Stimulation of the 7...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 1991
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.155.1.455